![]() Increasing evidence suggests that the differentiation of MSCs into adipocytes or osteoblasts is competitively balanced ( Li et al., 2016), and this delicate balance is important for the maintenance of bone homeostasis ( Hu et al., 2018b Muruganandan et al., 2020). In addition to osteoblasts, MSCs are also able to differentiate into adipocytes within bone marrow microenvironment. As the progenitor cells of osteoblasts and osteocytes, MSCs can migrate to defective sites and initiate new bone formation during the early stage of bone healing ( Tang et al., 2009). MSCs are important for preserving tissue homeostasis and have regeneration potential ( Bianco, 2014). Mesenchymal stem cells (MSCs) are multipotent stromal cells that originate from many connective tissues and can differentiate into a variety of cell types, such as osteoblasts, adipocytes, and myoblasts. These findings shed light on the contribution of mitochondrial dynamics to MSC fate and MSC-based tissue repair. We highlight mechanistic insights into modulating mitochondrial dynamics and mitochondrial strategies for stem cell-based regenerative medicine. This review focuses on the role of mitochondrial dynamics in MSC commitment under physiological and stress conditions. The coordination of mitochondrial fission and fusion is crucial for cellular function and stress responses, while abnormal fission and/or fusion causes MSC dysfunction. Emerging evidence suggests that mitochondrial dynamics are key contributors to stem cell fate determination. MSCs undergo specific mitochondrial dynamics during proliferation, migration, differentiation, apoptosis, or aging. Mitochondria are highly dynamic organelles that maintain their morphology via continuous fission and fusion, also known as mitochondrial dynamics. Mesenchymal stem cells (MSCs) are pivotal to tissue homeostasis, repair, and regeneration due to their potential for self-renewal, multilineage differentiation, and immune modulation. 3Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.2Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.1Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.This data will be used for regulatory network analysis and metabolic modeling to unravel the mechanisms and pathways involved, leading to a better understanding of mitochondrial dynamics in health and disease.Lin Ren 1,2,3†, Xiaodan Chen 1,2,3†, Xiaobing Chen 1,2,3, Jiayan Li 1,2,3, Bin Cheng 1,2,3* and Juan Xia 1,2,3* Moreover, we generated RNAseq data from cells in which established mitochondria dynamics proteins and novel candidate proteins were knocked down. In order to elucidate their exact role in mitochondrial dynamics, we perform functional experiments including gene-specific knockdown, assessment of mitochondrial morphology through fluorescent and electron microscopy, subcellular localization studies, assessment of mitochondrial activity, and many others. Through computational approaches based on protein-protein interactions, we identified several novel mitochondrial dynamics candidate proteins. The aim of our research is to identify and functionally characterize novel mitochondrial dynamics proteins. Although several mitochondrial dynamics proteins have been identified, not all involved proteins and mechanisms are known, restricting our understanding of disorders originating from a defect in mitochondrial dynamics. Their dynamic nature enables them to adapt their morphology to the physiological needs of the cell, through the process of fusion and fission, collectively referred to as mitochondrial dynamics. Mitochondria are dynamic organelles crucial for cellular energy production. ![]()
0 Comments
Leave a Reply. |